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蜂巢矩陣葉綠素熒光成像係統HEXAGON-IMAGING-PAM
日期:2022-08-31 09:30:40

蜂巢矩(ju)陣(zhen)葉(ye)綠(lv)素熒(ying)光(guang)成(cheng)像(xiang)係統

HEXAGON-IMAGING-PAM

葉綠素熒光成像係統(tong)的(de)六(liu)邊形(xing)戰(zhan)士(shi)

精(jing)度(du)高,麵(mian)積(ji)大,功(gong)能全,應用廣,文(wen)獻(xian)多(duo),數據(ju)可(ke)視(shi)化(hua)!

header-hexagon-imaging-pam-2560x1440-1086f31d.jpg

HEXAGON-IMAGING-PAM是(shi)德國(guo)WALZ公司(si)新推(tui)出的大型(xing)蜂巢矩陣葉綠素熒光成像係(xi)統。它(ta)憑(ping)借(jie)高(gao)精度的脈(mai)衝(chong)振幅(fu)調製(PAM)技術,可以對(dui)20×24cm的區域進(jin)行(xing)成像。分(fen)辨率(lv)高達(da)1.2 MP(1000 x 1200 px, 2x2 binning技術(shu),實(shi)際(ji)是2000×2400),像素(su)尺(chi)寸3.45 x 3.45 µm

超(chao)高分辨(bian)率的基(ji)礎(chu)是成像區域光場(chang)的均勻性,在設(she)計(ji)過(guo)程中(zhong),光源陣列中LED的位(wei)置(zhi)是經(jing)過精心(xin)布(bu)局(ju)的,以(yi)保(bao)證(zheng)測量區(qu)域內(nei)無(wu)陰(yin)影,所(suo)有成像區域內的樣(yang)品均勻(yun)照(zhao)光,樣品(pin)間的差異(yi)可以盡(jin)收(shou)眼(yan)底。大功率LED麵板的冷卻(que)效(xiao)果非常(chang)好(hao),可以大限(xian)度的延(yan)長LED的使(shi)用(yong)壽(shou)命(ming)。

增(zeng)加(jia)遠(yuan)紅(hong)光(FR)LED 麵板(ban),可用於測量(liang)所研(yan)究樣品的Fo'值(zhi)。

HEXAGON-IMAGING-PAM采(cai)用蜂(feng)巢(chao)矩陣式LED麵板拚(pan)接(jie)技(ji)術,單(dan)個(ge)六邊(bian)形蜂巢矩陣單元(yuan)之(zhi)間(jian)LED的不平(ping)橫(heng)可以獨(du)立(li)補(bu)償,初衷(zhong)是為實現(xian)樣品區域(yu)的理(li)想(xiang)照明提供(gong)更(geng)優(you)的選擇。

盡管(guan)成像區域很(hen)大,但(dan)是它依(yi)然足(zu)夠靈(ling)活(huo),可以測(ce)量各(ge)種(zhong)類型的樣品,如盆(pen)栽(zai)植物(wu),穴盤(pan)中培(pei)養的植(zhi)物,培養(yang)皿(min)上的植物或多孔板中的藻類(lei)懸(xuan)浮(fu)液(ye)。

滑(hua)動門設計,集(ji)成安全關閉(bi)功能,開門狀(zhuang)態下,飽(bao)和(he)脈衝的強度會被(bei)抑製以保護(hu)操作人(ren)員(yuan)的眼睛(jing)。

主(zhu)要(yao)功能

l 原(yuan)位測量:活體(ti)植物葉綠素熒光成像,直(zhi)觀(guan)顯示樣品光合作(zuo)用光能利(li)用差(cha)異,可導出彩(cai)色圖像。

l 成像功能:對Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、PS/50=ETR、Inh等參數(shu)進行成像分析。測定(ding)調節(jie)性(xing)能量耗散Y(NPQ),反映植物光保護能力(li),測定非(fei)調(diao)節性能量耗(hao)散(san)Y(NO),反(fan)映(ying)植物光損傷程度。

l 程序測量功能(neng):可自動(dong)程(cheng)序(xu)測量熒光誘(you)導曲線、快速(su)光曲(qu)線(xian)和暗(an)弛(chi)豫(yu),也(ye)可手(shou)動測量;在(zai)測量過程中能自動分析所有熒光參(can)數的變(bian)化趨(qu)勢;可以預(yu)編程進行自定義(yi)實驗(yan)流程,如(ru)模(mo)擬(ni)波(bo)動光。

l AOI功能:可在測量前或(huo)測量後(hou)任(ren)意選擇(ze)感(gan)興(xing)趣(qu)的區域(AOI),程序將(jiang)自動對選擇的AOI的數據進行變化趨勢(shi)分析,並在報(bao)告文件(jian)中顯(xian)示(shi)相(xiang)關(guan)AOI的數據。所有(you)報告文件中顯示的數據都(dou)可導(dao)出(chu)到(dao)EXCEL文件中。

l 成像異質(zhi)性分析功能:對任意(yi)參數任意時間的成像,可在圖像上(shang)任意選取(qu)兩(liang)點(dian),軟件自(zi)動對兩點(dian)間的數據進行橫向異質性分析,並(bing)可導出到EXCEL文件中。

l 成像數據範(fan)圍(wei)分析(xi)功能:對任意參數任意時間的成像,可分析任意兩個熒光數值之間有多少個像素點,多少(shao)麵積2

l 突變株(zhu)篩(shai)選功能:可跟(gen)據成像結果快(kuai)速篩選光合(he)、產氫/油(you)、抗逆(ni)(抗鹽(yan)、抗(kang)旱(han)、抗病等)等突變株。

l 微(wei)藻(zao)毒(du)理研究(jiu)功能:可同(tong)時(shi)測量4塊(kuai)96孔(kong)板,即(ji)384個微藻樣品(對照和處(chu)理組)的光合活性,軟(ruan)件自動給(gei)出處理組(zu)樣品相對於對照組的光合抑(yi)製(zhi)百(bai)分比(bi)。

7599516cab1fcc6b75f0f9ceaa70ce5.png

應(ying)用領域

l 光合作用研究:可以在完全相同的條(tiao)件下同時對大量樣品進行成像

l 植物病理學:病(bing)斑(ban)部(bu)位(包(bao)括(kuo)肉(rou)眼不(bu)可見時)成像以及(ji)病斑擴(kuo)散的時空(kong)動力學(xue)

l 植物脅(xie)迫(po)生理學:肉眼不可見(jian)生(sheng)物/非生物脅迫損(sun)傷(shang)的早(zao)期(qi)檢(jian)測

l 遺(yi)傳(chuan)育(yu)種:出苗(miao)後大規(gui)模快速篩選高光合/抗旱/抗熱/抗凍(dong)/抗病等(deng)植株

l 突變株篩選(xuan):快速篩選模式(shi)植物的光合突(tu)變株、抗逆突變株、產(chan)氫(qing)微藻突變株等

l 微藻毒理學:不同毒物濃(nong)度多個重(zhong)複(fu)的樣品一(yi)次(ci)測完(wan),軟件自動計算(suan)抑製比率

l 其(qi)它多種擴展(zhan)研究

成像參數

Fo, Fm, F, Ft, Fm', Fv/Fm, Y(II), qL, qP, qN, NPQ, Y(NPQ), Y(NO), PS/50=ETR,Inh.

imagingwingige-software-screenshot-753f5098.png


蜂巢矩陣葉綠素熒光成像係統視頻二維碼.png

產地(di):德(de)國WALZ


參考文獻

數據來源(yuan):光合作用文獻Endnote數據庫(ku),原始(shi)數據來源:Google Scholar

注(zhu):HEXAGON-IMAGING-PAM為(wei)新產品,暫無文獻發表(biao),研究成果(guo)可參考(kao)M-IMAGING-PAM發表文章。

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